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cr labeled yac 1 target cells  (ATCC)


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    ATCC cr labeled yac 1 target cells
    Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed <t>against</t> <t>YAC-1</t> target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.
    Cr Labeled Yac 1 Target Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cr labeled yac 1 target cells/product/ATCC
    Average 96 stars, based on 738 article reviews
    cr labeled yac 1 target cells - by Bioz Stars, 2026-06
    96/100 stars

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    1) Product Images from "CD127 + Natural Killer Cells Represent a Distinct, Interleukin-15-Independent and Thymus-Independent Subset in Mice"

    Article Title: CD127 + Natural Killer Cells Represent a Distinct, Interleukin-15-Independent and Thymus-Independent Subset in Mice

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms27062667

    Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed against YAC-1 target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.
    Figure Legend Snippet: Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed against YAC-1 target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.

    Techniques Used: Functional Assay, Activity Assay, Isolation, Release Assay, Two Tailed Test



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    Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed <t>against</t> <t>YAC-1</t> target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.
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    Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed <t>against</t> <t>YAC-1</t> target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.
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    Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed against YAC-1 target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.

    Journal: International Journal of Molecular Sciences

    Article Title: CD127 + Natural Killer Cells Represent a Distinct, Interleukin-15-Independent and Thymus-Independent Subset in Mice

    doi: 10.3390/ijms27062667

    Figure Lengend Snippet: Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed against YAC-1 target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.

    Article Snippet: Briefly, NK cell-mediated cytotoxicity was assessed by measuring 51 Cr-release following the incubation of freshly isolated, unstimulated effector cells with 51 Cr-labeled YAC-1 target cells (ATCC).

    Techniques: Functional Assay, Activity Assay, Isolation, Release Assay, Two Tailed Test